Standard Grade

High antiviral performance (proven effectiveness)

Eliminates 99.9% of enveloped viruses within one minute.*
* Under in vitro conditions * Not necessarily effective against all types of bacteria and viruses

Antiviral effectiveness

Test data for virus A (enveloped virus) verified by Japan Textile Products Quality and Technology Center

Test outline
Test organization Japan Textile Products Quality and Technology Center
Test method Antiviral test (plaque measurement method)
Test virus Virus A
Samples Standard Grade functional reducing ionized water, reference solution (phosphate buffered saline)

Results

Viral infectivity (amount of particulate viruses with cell infectivity) was evaluated after mixing 1.9 ml of Standard Grade functional reducing ionized water with 0.1 ml of virus solution for 1 and 5 minutes. Figure 1 shows plaque measurement results after halting the test solution reaction with a chemical deactivator, then preparing a series of 10× dilutions with a cell culture solution. The test shows Standard Grade functional reducing ionized water reduces virus counts by at least three orders of magnitude (99.9%) when brought into contact with virus A for one minute.
(a) Reference solution (phosphate buffered saline)

Referred to as plaque, the white spots visible in the figure indicate where virus infections have destroyed cells. Plaques overlap when large numbers of viruses are destroyed. The test solution is diluted after halting the reaction to allow accurate counts of the plaque. The plaque count, dilution, and virus concentration of the test solution are then used to calculate viral infectivity (plaque measurement method).

Figure 1: Virus A plaque measurement results for reference solution (PBS)

(b) Standard Grade functional reducing ionized water

Figure 2: Virus A plaque measurement results after exposure to Standard Grade functional reducing ionized water



Figure 3: Infectivity reduction graph for virus A and Standard Grade functional reducing ionized water

Eliminates 99.9% of bacteria*
(proven effectiveness)
* Under in vitro conditions * Not necessarily effective against all types of bacteria and viruses

Antibacterial effectiveness

Test data for bacteria A, B, and C verified by Union Bitech Co., Ltd..

Test outline
Test organization Union Bitech Co., Ltd.
Test method Test bacteria suspension method
Test bacteria Bacteria A, B, and C
Samples Standard Grade functional reducing ionized water, reference solution (phosphate buffered saline)

Results

A mixture of 19.8 g of Standard Grade functional reducing ionized water (test solution) and 0.2 ml (0.1% vol) of bacteria suspension solution was adjusted to achieve a bacterial count of approximately 108 per ml. This mixture was then allowed to react for a specified duration. The test solution was then diluted and the reaction deactivated. Graded dilutions were used to count the number of bacteria (colonies) by the petriplate method. The results show exposing three different bacteria types (A, B, and C) to the test solution reduces bacteria counts by at least three orders of magnitude (99.9%) compared to the reference solution. (Figure 4)

Figure 4: Bacterial reduction graph for Standard Grade functional reducing ionized water

* URL for product development partner A.I.System Products Corporation’s home page:
http://www.aisp.co.jp/