Premium Grade
High antibacterial and antiviral performance (proven effectiveness)
Eliminates 99.9% of enveloped and non-enveloped viruses and bacteria within 20 seconds*
* Under in vitro conditions * Not necessarily effective against all types of bacteria and viruses
Antiviral effectiveness
Test data for virus A (enveloped virus) verified by Japan Textile Products Quality and Technology Center
Test outline
| Test organization |
Japan Textile Products Quality and Technology Center |
| Test method |
Antiviral test (plaque measurement method) |
| Test virus |
Virus A |
| Samples |
Premium Grade functional reducing ionized water, reference solution (phosphate buffered saline) |
Results
Viral infectivity (amount of particulate viruses with cell infectivity) was evaluated after mixing 1.9 ml of Premium Grade functional reducing ionized water with 0.1 ml of virus solution for durations of 20 seconds and 1 minute. Figure 1 shows plaque measurement results after halting the test solution reaction with a chemical deactivator, then preparing a series of 10× dilutions with a cell culture solution. The test shows Premium Grade functional reducing ionized water reduces virus counts by at least four orders of magnitude (99.99%) when brought into contact with virus A for 20 seconds. (Figure 2)
(a) Reference solution (phosphate buffered saline)
Referred to as plaque, the white spots visible in the figure indicate where virus infections have destroyed cells. Plaques overlap when large numbers of viruses are destroyed. The test solution is diluted after halting the reaction to allow accurate counts of the plaque. The plaque count, dilution, and virus concentration of the test solution are then used to calculate viral infectivity (plaque measurement method).
Figure 1: Virus A plaque measurement results for reference solution (PBS)
(b) Premium Grade functional reducing ionized water
Figure 2: Virus A plaque measurement results after exposure to Premium Grade functional reducing ionized water
Figure 3: Infectivity reduction graph for virus A and Premium Grade functional reducing ionized water
Eliminates 99.9% of all bacteria within 30 seconds.*
(proven effectiveness)
* Under in vitro conditions * Not necessarily effective against all types of bacteria and viruses
Antibacterial effectiveness
Test data for bacteria A, B, and C verified by Union Bitech Co., Ltd..
Test outline
| Test organization |
Union Bitech Co., Ltd. |
| Test method |
Test bacteria suspension method |
| Test bacteria |
Bacteria A, B, and C |
| Samples |
Premium Grade functional reducing ionized water, reference solution (phosphate buffered saline) |
Results
A mixture of 19.8 g of Premium Grade functional reducing ionized water (test solution) and 0.2 ml (0.1% vol) of bacteria suspension solution was adjusted to achieve a bacterial count of approximately 108 per ml. This mixture was then allowed to react for a specified duration. The test solution was then diluted and the reaction deactivated. Graded dilutions were used to count the number of bacteria (colonies) by the petriplate method. The results show exposing three different bacteria types (A, B, and C) to the test solution reduces bacteria counts by at least three orders of magnitude (99.9%) within 30 seconds compared to the reference solution. (Figure 4)
Figure 4: Bacterial reduction graph for Premium Grade functional reducing ionized water
* URL for product development partner A.I.System Products Corporation’s home page:
http://www.aisp.co.jp/
